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Architecture of mixed-species biofilms exposed to tested substances. Representative 3-D renderings of biofilms at 67 h of development. Green represents bacterial cells labeled with SYTO 9, and red represents the exopolysaccharides stained with Alexa <t>Fluor</t> <t>647-labeled</t> dextran conjugate. The imaging was performed using a Zeiss LSM 780 AxioObserver microscope equipped with a 20×/0.5 objective lens. The larger image in each set represents the overlaid images of the red and green channels. The scale bar corresponds to 50 μm. Control: no substance.
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Architecture of mixed-species biofilms exposed to tested substances. Representative 3-D renderings of biofilms at 67 h of development. Green represents bacterial cells labeled with SYTO 9, and red represents the exopolysaccharides stained with Alexa <t>Fluor</t> <t>647-labeled</t> dextran conjugate. The imaging was performed using a Zeiss LSM 780 AxioObserver microscope equipped with a 20×/0.5 objective lens. The larger image in each set represents the overlaid images of the red and green channels. The scale bar corresponds to 50 μm. Control: no substance.
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Architecture of mixed-species biofilms exposed to tested substances. Representative 3-D renderings of biofilms at 67 h of development. Green represents bacterial cells labeled with SYTO 9, and red represents the exopolysaccharides stained with Alexa <t>Fluor</t> <t>647-labeled</t> dextran conjugate. The imaging was performed using a Zeiss LSM 780 AxioObserver microscope equipped with a 20×/0.5 objective lens. The larger image in each set represents the overlaid images of the red and green channels. The scale bar corresponds to 50 μm. Control: no substance.
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Architecture of mixed-species biofilms exposed to tested substances. Representative 3-D renderings of biofilms at 67 h of development. Green represents bacterial cells labeled with SYTO 9, and red represents the exopolysaccharides stained with Alexa <t>Fluor</t> <t>647-labeled</t> dextran conjugate. The imaging was performed using a Zeiss LSM 780 AxioObserver microscope equipped with a 20×/0.5 objective lens. The larger image in each set represents the overlaid images of the red and green channels. The scale bar corresponds to 50 μm. Control: no substance.
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Architecture of mixed-species biofilms exposed to tested substances. Representative 3-D renderings of biofilms at 67 h of development. Green represents bacterial cells labeled with SYTO 9, and red represents the exopolysaccharides stained with Alexa Fluor 647-labeled dextran conjugate. The imaging was performed using a Zeiss LSM 780 AxioObserver microscope equipped with a 20×/0.5 objective lens. The larger image in each set represents the overlaid images of the red and green channels. The scale bar corresponds to 50 μm. Control: no substance.

Journal: ACS Omega

Article Title: Putative Prebiotics Can Disrupt 3D Architecture and Modulate the Microbial Population to Prevent Cariogenic Biofilm Build-Up In Vitro

doi: 10.1021/acsomega.5c11573

Figure Lengend Snippet: Architecture of mixed-species biofilms exposed to tested substances. Representative 3-D renderings of biofilms at 67 h of development. Green represents bacterial cells labeled with SYTO 9, and red represents the exopolysaccharides stained with Alexa Fluor 647-labeled dextran conjugate. The imaging was performed using a Zeiss LSM 780 AxioObserver microscope equipped with a 20×/0.5 objective lens. The larger image in each set represents the overlaid images of the red and green channels. The scale bar corresponds to 50 μm. Control: no substance.

Article Snippet: Biofilms were prepared (as described above) with the addition of a 1 μM Alexa Fluor 647-labeled dextran conjugate (absorbance/fluorescence emission maxima of 647/668 nm; Molecular Probes, Carlsbad, CA, USA) to the culture medium throughout the experiment.

Techniques: Labeling, Staining, Imaging, Microscopy, Control

Architecture of S. mutans biofilms exposed to tested substances. Representative 3-D renderings of biofilms at 67 h of development. Green represents bacterial cells labeled with SYTO 9, and red represents the exopolysaccharides stained with Alexa Fluor 647-labeled dextran conjugate. The imaging was performed using a Zeiss LSM 780 AxioObserver microscope equipped with a 20×/0.5 objective lens. The larger image in each set represents the overlaid red and green channels. The scale bar corresponds to 50 μm. Control: no substance.

Journal: ACS Omega

Article Title: Putative Prebiotics Can Disrupt 3D Architecture and Modulate the Microbial Population to Prevent Cariogenic Biofilm Build-Up In Vitro

doi: 10.1021/acsomega.5c11573

Figure Lengend Snippet: Architecture of S. mutans biofilms exposed to tested substances. Representative 3-D renderings of biofilms at 67 h of development. Green represents bacterial cells labeled with SYTO 9, and red represents the exopolysaccharides stained with Alexa Fluor 647-labeled dextran conjugate. The imaging was performed using a Zeiss LSM 780 AxioObserver microscope equipped with a 20×/0.5 objective lens. The larger image in each set represents the overlaid red and green channels. The scale bar corresponds to 50 μm. Control: no substance.

Article Snippet: Biofilms were prepared (as described above) with the addition of a 1 μM Alexa Fluor 647-labeled dextran conjugate (absorbance/fluorescence emission maxima of 647/668 nm; Molecular Probes, Carlsbad, CA, USA) to the culture medium throughout the experiment.

Techniques: Labeling, Staining, Imaging, Microscopy, Control